KMID : 0545120060160101570
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Journal of Microbiology and Biotechnology 2006 Volume.16 No. 10 p.1570 ~ p.1576
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Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea
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Song Min-Suk
Joo Young-Ho Lee Eun-Ho Shin Jin-Young Kim Chul-Jung Shin Kwang-Soon Sung Moon-Hee Choi Young-Ki
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Abstract
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An encephalomyocarditis virus (EMCV-CBNU) was isolated from an aborted swine fetus in October 2005. To investigate the genetic origin and virulence of the EMCV-CBNU strain, we determined the complete sequence of the virus and tested its virulence in mice. Genetic characterization revealed that the RNA genome was composed of 7,713 nucleotides with a single open reading frame (2,292 amino acids), coding 12 proteins. The EMCV-CBNU had the shortest poly(C) tract, consisting of 10 C's (), compared with all the other EMCV strains reported in GenBank. Amino acid and phylogenetic analyses showed that EMCV-CBNU had the highest genetic identity with strain 2887A (99.7%), which was originally isolated from a fetus in a pig breeding farm that had a history of reproductive failure. Because rodents are the natural host of EMCV, we investigated the virulence of EMCV-CBNU in mice. Surprisingly, all mice inoculated with more than of EMCV-CBNU showed symptoms of hind limb paralysis and eventually died during 3 and 8 days postinoculation (DPI). Furthermore, when we inoculated the virus into pregnant mice, all dams and their fetuses died in 6 DPI. This is the first report on a full genomic analysis of swine EMCV in Korea, which exhibits high virulence in mice.
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KEYWORD
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Swine, encephalomyocarditis virus, reproductive failure, mice
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